{"id":262,"date":"2020-11-04T18:04:24","date_gmt":"2020-11-04T09:04:24","guid":{"rendered":"http:\/\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/?page_id=262"},"modified":"2021-05-19T11:38:17","modified_gmt":"2021-05-19T02:38:17","slug":"live-imaging","status":"publish","type":"page","link":"https:\/\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/?page_id=262&lang=en","title":{"rendered":"Live-imaging of early embryogenesis"},"content":{"rendered":"\n<p><\/p>\n\n\n\n<div class=\"wp-block-media-text alignwide is-stacked-on-mobile\" style=\"grid-template-columns:43% auto\"><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"295\" height=\"135\" src=\"https:\/\/i0.wp.com\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/wp-content\/uploads\/2021\/05\/image2-1.png?resize=295%2C135\" alt=\"\" class=\"wp-image-775\" data-recalc-dims=\"1\"\/><\/figure><div class=\"wp-block-media-text__content\">\n<p class=\"has-text-align-justify has-normal-font-size\">Plants have versatile morphologies and create complex organs including flowers, leaves, roots and stems. The basis of morphology formation&nbsp; is to establish proper orientation known as the body axis. In most plants, fertilized eggs divide asymmetrically to establish the vertical axis (Figure). However, since fertilized eggs of flowering plants develop deeply within flowers or seeds, how the fertilized egg becomes polarized and how it divides asymmetrically remains to be answered.<\/p>\n<\/div><\/div>\n\n\n\n<div style=\"height:27px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"has-text-align-justify has-normal-font-size\">Using <em>Arabidopsis thaliana<\/em>, we successfully observed the internal structure of fertilized plant eggs in real time. We discovered the spatiotemporal dynamics of cytoskeleton and organelles in fertilized eggs, which contribute to the polarity establishment. For example, before fertilization, microtubules are oriented along the vertical axis, but this alignment collapses upon fertilization. The microtubules then form a ring-like structure that moves along the elongating zygotes. On the other hand, actin fibers are oriented along the vertical axis and are responsible for the apical movement of the nucleus.<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-layout-1 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<iframe loading=\"lazy\" width=\"560\" height=\"315\" src=\"https:\/\/www.youtube.com\/embed\/eon2M8PkINo?autoplay=1&amp;loop=1&amp;mute=1&amp;playlist=eon2M8PkINo\" frameborder=\"0\" allow=\"accelerometer; clipboard-write; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen=\"\"><\/iframe>\n\n\n\n<p><strong>Live-imaging of microtubules in zygote<\/strong><br>Kimata et al. (2016),  Movie S1<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"286\" height=\"219\" src=\"https:\/\/i0.wp.com\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/wp-content\/uploads\/2021\/05\/image3.png?resize=286%2C219\" alt=\"\" class=\"wp-image-774\" data-recalc-dims=\"1\"\/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:36px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"has-text-align-justify has-normal-font-size\">The vacuole, which makes up the majority of plant cells\u2019 volume, shrinks rapidly after fertilization. The vacuole then forms filamentous tubular structures around the nucleus and gradually moves toward the basal end. In <em>sgr2<\/em> mutant plants, where the flexibility of the vacuole is reduced, the tubular structure is not formed and the vacuole cannot move toward the basal end. As a result, a large vacuole is left at the apical end of the fertilized egg and prevents the nucleus from reaching the apical end, resulting in a near symmetrical division. This indicates that&nbsp; the flexible vacuole plays a role in asymmetric division by changing its shape and moving toward the basal end to support the movement of the nucleus to the opposite side.<\/p>\n\n\n\n<p class=\"has-text-align-justify has-normal-font-size\">When a fertilized egg divides asymmetrically, it produces a smaller apical cell (precursor of embryo proper) with few vacuoles and a larger basal cell (precursor of suspensor) with large vacuoles. In the <em>sgr2<\/em> mutant plants, both the apical and basal cells are similar in size and inherit large vacuoles. As embryogenesis proceeds, the giant vacuoles remain in the embryo proper, impair embryonic patterning and ultimately result in an abnormal number of cotyledons. It is known that actively dividing cells contain few vacuoles in plants, so the abnormal embryonic morphology in <em>sgr2<\/em> mutant plants is thought to be a result of the inheritance of excess vacuoles in the apical cell.<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-layout-2 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<iframe loading=\"lazy\" width=\"560\" height=\"315\" src=\"https:\/\/www.youtube.com\/embed\/ZcQBf9ruWfk?autoplay=1&amp;loop=1&amp;mute=1&amp;playlist=ZcQBf9ruWfk\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen=\"\"><\/iframe>\n\n\n\n<p><strong>Live-imaging of vacuoles in wild type zygote<\/strong><br>Kimata et al. (2019), Movie S1<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<iframe loading=\"lazy\" width=\"560\" height=\"315\" src=\"https:\/\/www.youtube.com\/embed\/uSWR4KMEb30?autoplay=1&amp;loop=1&amp;mute=1&amp;playlist=uSWR4KMEb30\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen=\"\"><\/iframe>\n\n\n\n<p><strong>Live-imaging of vacuoles in <em>sgr2<\/em> zygote<\/strong><br>Kimata et al. (2019), Movie S2<\/p>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"has-text-align-justify has-normal-font-size\">The discovery that vacuoles actively contribute to cell polarization and embryonic morphology has overturned the conventional impression of vacuoles as mere passive &#8220;water bags&#8221;. Therefore, we aim to overcome the limitations of conventional research methods and elucidate the developmental mechanisms at the intracellular level without preconceptions by performing precise live imaging of various intracellular structures and determining their roles using inhibitors or mutants.<\/p>\n\n\n\n<div style=\"height:38px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><span style=\"text-decoration: underline\" class=\"underline\">References<\/span><br>Kimata et al. (2016) Proc Nat Acad Sci 113 (49), pp14157-14162, DOI:<a rel=\"noreferrer noopener\" href=\"https:\/\/dx.doi.org\/10.1073\/pnas.1613979113\" target=\"_blank\">10.1073\/pnas.1613979113<\/a><br>Kurihara et al. (2017) JoVE (127), 55975, DOI:&nbsp;<a rel=\"noreferrer noopener\" href=\"https:\/\/dx.doi.org\/10.3791\/55975\" target=\"_blank\">10.3791\/55975<\/a><br>Kimata et al. (2019) Proc Nat Acad Sci 116 (6), pp2338-2343,&nbsp;DOI:&nbsp;<a rel=\"noreferrer noopener\" href=\"https:\/\/dx.doi.org\/10.1073\/pnas.1814160116\" target=\"_blank\">10.1073\/pnas.1814160116<\/a><br>Kimata et al., (2020) Quant Plant Biol 1, e3, DOI:&nbsp;<a href=\"https:\/\/doi.org\/10.1017\/qpb.2020.4\" target=\"_blank\" rel=\"noreferrer noopener\" title=\"https:\/\/doi.org\/10.1017\/qpb.2020.4\">10.1017\/qpb.2020.4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Plants have versatile morpholo &#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":220,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":"","_locale":"en_US","_original_post":"http:\/\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/?page_id=99"},"aioseo_notices":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/index.php?rest_route=\/wp\/v2\/pages\/262"}],"collection":[{"href":"https:\/\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=262"}],"version-history":[{"count":5,"href":"https:\/\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/index.php?rest_route=\/wp\/v2\/pages\/262\/revisions"}],"predecessor-version":[{"id":799,"href":"https:\/\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/index.php?rest_route=\/wp\/v2\/pages\/262\/revisions\/799"}],"up":[{"embeddable":true,"href":"https:\/\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/index.php?rest_route=\/wp\/v2\/pages\/220"}],"wp:attachment":[{"href":"https:\/\/www.lifesci.tohoku.ac.jp\/PlantCellDyn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=262"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}