Assistant Professor KUCHITSU Yoshihiko
| Campus | Aobayama campus |
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| Laboratory |
Organelle Pathophysiology
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| Tel | +81-22-795-6684 |
| yoshihiko.kuchitsu.d8＠tohoku.ac.jp |
| Career |
2017: Tohoku University, Department of Biology, Faculty of Science
2019: Tohoku University, Graduate School of Life Sciences 2022: Tohoku University, Graduate School of Life Sciences 2019: JSPS Research Fellow (DC1)
2022: Researcher, Graduate School of Life Sciences, Tohoku University 2023: JSPS Research Fellow (PD) 2024: Current Position |
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| Selected Publications |
Original paper
Kemmoku, H., Takahashi, K., Mukai, K., Mori, T., Hirosawa, K. M., Kiku, F., Uchida, Y., Kuchitsu, Y., Nishioka, Y., Sawa, M., Kishimoto, T., Tanaka, K., Yokota, Y., Arai, H., Suzuki, K. G. N. and Taguchi, T. (2024) Single-molecule localization microscopy reveals STING clustering at the trans-Golgi network through palmitoylation-dependent accumulation of cholesterol. Nat Commun 15, 220 Kuchitsu, Y.*, Mukai, K.*, Uematsu, R., Takaada, Y., Shinojima, A., Shindo, R., Shoji, T., Hamano, S., Ogawa, E., Sato, R., Miyake, K., Kato, A., Kawaguchi, Y., Nishitani-Isa, M., Izawa, K., Nishikomori, R., Yasumi, T., Suzuki, T., Dohmae, N., Uemura, T., Barber, G. N., Arai, H., Waguri, S. and Taguchi, T. (2023) STING signalling is terminated through ESCRT-dependent microautophagy of vesicles originating from recycling endosomes. Nat. Cell Biol. 25, 453-466 (*equally contributed author)
Shindo, R. *, Kuchitsu, Y. *, Mukai, K. and Taguchi, T. (2022) The activity of disease-causative STING variants can be suppressed by wild-type STING through heterocomplex formation. Front Cell Dev Biol 10, 1037999 (*equally contributed author)
Kemmoku, H.*, Kuchitsu, Y.*, Mukai, K. and Taguchi, T. (2022) Specific association of TBK1 with the trans-Golgi network following STING stimulation. Cell Struct. Funct. 47, 19-30 (*equally contributed author)
Mukai, K., Ogawa, E., Uematsu, R., Kuchitsu, Y., Kiku, F., Uemura, T., Waguri, S., Suzuki, T., Dohmae, N., Arai, H., Shum, A. K. and Taguchi, T. (2021) Homeostatic regulation of STING by retrograde membrane traffic to the ER. Nat Commun 12, 61
Homma, Y., Kinoshita, R., Kuchitsu, Y., Wawro, P. S., Marubashi, S., Oguchi, M. E., Ishida, M., Fujita, N. and Fukuda, M. (2019) Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218, 2035-2050
Zhu, S., Bhat, S., Syan, S., Kuchitsu, Y., Fukuda, M. and Zurzolo, C. (2018) Rab11a-Rab8a cascade regulates the formation of tunneling nanotubes through vesicle recycling. J. Cell Sci. 131, jcs215889
Kuchitsu, Y., Homma, Y., Fujita, N. and Fukuda, M. (2018) Rab7 knockout unveils regulated autolysosome maturation induced by glutamine starvation. J. Cell Sci. 131, jcs215442
Fujita, N., Huang, W., Lin, T. H., Groulx, J. F., Jean, S., Nguyen, J., Kuchitsu, Y., Koyama-Honda, I., Mizushima, N., Fukuda, M. and Kiger, A. A. (2017) Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy. Elife 6, e23367
Review Kuchitsu, Y. and Taguchi, T. (2024) STINGing organelle surface with acid. EMBO Rep. 25: 1708 - 1710 Kuchitsu, Y. and Taguchi, T. (2023) Lysosomal microautophagy: an emerging dimension in mammalian autophagy. Trends Cell Biol. 34, 7, 606-616
Kuchitsu, Y. and Fukuda, M. (2018) Revisiting Rab7 Functions in Mammalian Autophagy: Rab7 Knockout Studies. Cells 7, 215
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Recent Activities
I am investigating the mechanisms of intracellular protein degradation. Our bodies are composed of approximately 30 trillion cells, each harboring about 20,000 different types of proteins. Proteins that are aged or no longer needed are degraded in lysosomes, which function as the cell's "recycling centers." My research has focused on the degradation mechanism of a protein called STING, which is activated during viral infections, within lysosomes. Utilizing advanced imaging techniques such as super-resolution and electron microscopy, I captured the dynamic process of lysosomes moving within the cell, directly engulfing and degrading STING. Lysosomes have been viewed as static "recycling centers" where waste is passively transported for degradation. However, my findings reveal that lysosomes actively traverse the cellular environment, functioning akin to "garbage trucks" that collect cellular debris. Currently, I am dedicated to elucidating the novel functions of lysosomes and their broader implications in cellular homeostasis.