Integrative Life Sciences :
Developmental Regulation Network


Assistant Professor HAYASHI Yohei
Campus Seiryo campus
Laboratory Germ Cell Development
Tel +81-22-717-8572
E-mail yohei.hayashi.e2@tohoku.ac.jp
Website http://www2.idac.tohoku.ac.jp/dep/crcbr/

In graduate school, we worked on the elucidation of epigenetic mechanisms for the regulation of gene expression in budding yeast. Currently, I am attracted to study of the germ cells responsible for intergenerational information inheritance. I am interested in how the information possessed by germ cells is changed or inherited through dynamic phenomena such as germ-cell differentiation, fertilization, and ontogeny.

2005 Graduated Faculty of Pharmaceutical Science, University of Tokyo
2010 Completed Graduate School of Science, University of Tokyo
2011 Post-doctoral Fellow, Institute of Molecular and Cellular Biosciences, University of Tokyo
2011-2014 Post-doctoral Fellow, Graduate School of Pharmaceutical Science, University of Tokyo
2014- Current position
Selected Publications
  1. Mochizuki K*, Hayashi Y*, Sekinaka T*, Otsuka K, Ito-Matsuoka Y, Kobayashi H, Oki S, Takehara A, Kono T, Osumi N, Matsui Y. Repression of Somatic Genes by Selective Recruitment of HDAC3 by BLIMP1 Is Essential for Mouse Primordial Germ Cell Fate Determination. Cell Rep. 24, 2682-2693 (2018). (*equally contributed)
  2. Hayashi Y, Matsui Y. Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells.
    Methods Mol. Biol. doi: 10.1007/7651_2018_164 (2018).
  3. Hayashi Y, Otsuka K*, Ebina M*, Igarashi K*, Takehara A, Matsumoto M, Kanai A, Igarashi K, Soga T, Matsui Y. Distinct requirements for energy metabolism in mouse primordial germ cells and their reprogramming to embryonic germ cells. Proc. Natl. Acad. Sci. U.S.A. 114, 8289-8294 (2017). (*equally contributed)
  4. Sekinaka T, Hayashi Y, Noce T, Niwa H, Matsui Y. Selective de-repression of germ cell-specific genes in mouse embryonic fibroblasts in a permissive epigenetic environment. Sci Rep., 6, 32932 (2016).
  5. Matsumoto Y, Yasukawa J, Ishii M, Hayashi Y, Miyazaki S, Sekimizu K. A critical role of mevalonate for peptidoglycan synthesis in Staphylococcus aureus. Sci Rep., 6, 22894 (2016).
  6. Matsumoto Y, Ishii M, Hayashi Y, Miyazaki S, Sugita T, Sumiya E, Sekimizu K. Diabetic silkworms for evaluation of therapeutically effective drugs against type II diabetes. Sci Rep., 5, 10722 (2015).
  7. Adachi T, Ishii K, Matsumoto Y, Hayashi Y, Hamamoto H, Sekimizu K. Niemann-Pick disease type C2 protein induces triglyceride accumulation in silkworm and mammalian cell lines.
    Biochem J., 459, 137-147 (2014).
  8. Kawano A, Hayashi Y, Noguchi S, Handa H, Horikoshi M, Yamaguchi Y. Global analysis for functional residues of histone variant Htz1 using the comprehensive point mutant library.
    Genes Cells, 16, 590-607, (2011).
  9. Sato L*, Noguchi S*, Hayashi Y*, Sakamoto M*, Horikoshi M. Global analysis of functional relationships between histone point mutations and the effects of histone deacetylase inhibitors.
    Genes Cells, 15, 553-594, (2010) (*equally contributed).
  10. Akai Y*, Adachi N*, Hayashi Y*, Eitoku M, Sano N, Natsume R, Kudo N, Tanokura M, Senda T, Horikoshi M. Structure of the histone chaperone CIA/ASF1-double bromodomain complex linking histone modifications and site-specific histone eviction. Proc. Natl Acad. Sci. U.S.A., 107, 8153-8158, (2010) (*equally contributed).
  11. Hayashi Y* , Senda T*, Sano N*, Horikoshi M. Theoretical framework for the histone modification network: modifications in the unstructured histone tails form a robust scale-free network. Genes Cells, 14, 789-806, (2009) (*equally contributed).
Activities in Academic Societies

The Molecular Biology Society of Japan, Japanese Society of Developmental Biologists

Recent Activities

I started researching the metabolic regulation of germline development. It is becoming clear that metabolism is indispensable for maintaining life in many organisms and cell types and its regulation affects the determination of cell fates. Recent research topics are how the metabolic activity is controlled during germline development, and how the metabolic regulation of germ cells affects their reproductive functions for inheriting genetic and epigenetic information to the next generation.

Metabolic analysis in a small number of cells like germ cells is almost unprecedented, so I am tacking on challenging experiments everyday.

Message to Students

I think the best part of research is the course of setting up a problem on your own, formulating a hypothesis, and solving the problem with logic, experiments, and discussions. It becomes addictive to discover the laws of nature by repeating this process. Please nurture the seeds of your curiosity and grow buds of discovery. Let's grow them together if there is a chance for you to research with me.