Integrative Life Sciences :
Brain and Nervous System


Assistant Professor ANSAI Satoshi
Campus Katahira campus
Laboratory Molecular Ethology
Tel +81-22-217-6219
E-mail satoshi.ansai.e7@tohoku.ac.jp
Website http://satoshi-ansai.github.io/
Google Scholar
  I mainly study medaka and its related species as model systems. Recently, I am focusing on medaka fishes endemic to Sulawesi, Indonesia, to understand the molecular/neural mechanisms of phenotypic diversification in a closely-related species complex. I am also interested in genomics of medaka-related species and development of genetic engineering techniques including targeted genome editing in medaka.
2011          Bachelor, Department of Bioresources, Faculty of Agriculture, Kyoto University
2013          Master, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
2016          Ph.D., Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
2016–2017 JSPS Research Fellowship for Young Scientists (PD), Division of Ecological Genetics, Department of Population Genetics, National Institute of Genetics
2017–2019 Assistant Professor, Laboratory of Bioresources, National Institute for Basic Biology
2019–        Assistant Professor, Graduate School of Life Siences, Tohoku University
Selected Publications
1. Ishikawa T*, Ansai S*, Kinoshita M, Mori K. (2018) A Collection of Transgenic Medaka Strains for Efficient Site-Directed Transgenesis Mediated by phiC31 Integrase. G3: Genes, Genomes, Genetics, 8: 2585-2593. (*equally contributed)
2. Ansai S, Hosokawa H, Maegawa S, Naruse K, Washio Y, Sato K, Kinoshita M. (2017) Deficiency of serotonin in raphe neurons and altered behavioral responses in tryptophan hydroxylase 2-knockout medaka (Oryzias latipes). Zebrafish, 14: 495-507.
3. Murakami Y, Ansai S, Yonemura A, Kinoshita M. (2017) An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes). Zool. Lett., 3: 10.
4. Ansai S, Hosokawa H, Maegawa S, Kinoshita M. (2016) Chronic fluoxetine treatment induces anxiolytic responses and altered social behaviors in medaka, Oryzias latipes. Behav. Brain Res., 303C: 126-136.
5. Ansai S, Kinoshita M. (2014) Targeted mutagenesis using CRISPR/Cas system in medaka. Biol. Open, 3: 362-371.
6. Ansai S, Inohaya K, Yoshiura Y, Schartl M, Uemura N, Takahashi R, Kinoshita M. (2014) Design, evaluation, and screening methods for efficient targeted mutagenesis with TALENs in medaka. Dev., Growth Differ. 56: 98-107.
7. Ansai S, Sakuma T, Yamamoto T, Ariga H, Uemura N, Takahashi R, Kinoshita M. (2013) Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases (TALENs). Genetics, 193: 739-749.
8. Ansai S, Ochiai H, Kanie Y, Kamei Y, Gou Y, Kitano T, Yamamoto T, Kinoshita M. (2012) Targeted disruprion of exogenous EGFP gene in medaka using zinc-finger nucleases. Dev. Growth Differ., 54: 546-556.
Activities in Academic Societies
The Zoological Society of Japan, Society of Evolutionary Studies Japan, The Ecological Society of Japan, The Japanese Society of Fisheries Science

Recent Activities

  We are studying on the molecular basis of diversification in male nuptial colorations of Sulawesi-endemic medaka species. A causal gene for red pectoral fins in Oryzias woworae males was identified by genetic analysis. Then, we showed that the red coloration in males affected the female mate preference by behavioral analysis using mutant fish for the causal gene.

Message to Students

  I want to understand how organisms can evolve and become so diversified by both genetic engineering technologies in laboratory and field sampling in wild. If you are interested in these kinds of researches, please feel free to contact me.