｜Graduate School of Life Sciences Tohoku University

Field

Molecular and Chemical Life Science :
Molecular and Network Genomics

Research

JAPANESE

Assistant Professor KISHIDA Kouhei

Campus	Katahira campus
Laboratory	Microbial Genetics and Evolution
Tel	+81-22-217-5696
E-mail	kouhei.kishida.a7＠tohoku.ac.jp
Website	http://www.lifesci.tohoku.ac.jp/biideshin/index.html

Google scholar

https://scholar.google.co.jp/citations?hl=ja&user=H0YyAoUAAAAJ

Researchmap

https://researchmap.jp/kishida_plasmid

I became interested in biology after learning about the endosymbiotic theory in my high school biology class.

Career	2012 B.A. in Science, the Department of Science, Ibaraki University 2014 MSc in Life Science, Graduate School of Life Sciences, Tohoku University 2017 PhD in Life Science, Graduate School of Life Sciences, Tohoku University 2017-19 Postdoctoral Researcher in Tohoku University 2019-23 Postdoctoral Researcher in UThealth 2023- Assistant Professor in Tohoku University
Selected Publications	Al Mamun AAM, K Kissoon, Kishida K, Shropshire W, Hanson B, Christie PJ. 2022 IncFV plasmid pED208: Sequence analysis and evidence for translocation of maintenance/leading region proteins through diverse type IV secretion systems. Plasmid, 123: 102652 Kishida K, Bosserman RE, Harb L, Khara P, Song L, Hu B, Zeng L, Christie PJ. 2021 Contributions of F‐specific Subunits to the F Plasmid‐Encoded Type IV Secretion System and F pilus. Mol. Microbiol, 117:1275-1290 Al Mamun AAM, Kishida K, Christie PJ. 2021 Protein Transfer through an F Plasmid-Encoded Type IV Secretion System Suppresses the Mating-Induced SOS Response. *mbio, 12: e01629-21 Nonoyama S, Kishida K, Sakai K, Nagata Y, Ohtsubo Y, Tsuda M. 2020 A transcriptional regulator, IscR, of Burkholderia multivorans* acts as both repressor and activator for transcription of iron-sulfur cluster-biosynthetic isc operon. Research in microbiology, 171: 319-330 Kishida K, Nonoyama S, Lucas T, Kawahara S, Kudo K, Nagata Y, Ohtsubo Y, Tsuda M. 2019 Conjugative Transfer of IncP-9 Catabolic Plasmids Requires a Previously Uncharacterized Gene mpfK, Whose Homologs Are Conserved in Various MPFT-Type Plasmids. Appl Environ Microbiol, 85: e01850-19 Ogawa N, Kato H, Kishida K, Ichihashi E, Ishige T, Yoshikawa H, Nagata Y, Ohtsubo Y, Tsuda M. 2019 Suppression of substrate inhibition in phenanthrene-degrading Mycobacterium by co-cultivation with a non-degrading Burkholderia strain. *Microbiology, 165: 625-637 Kishida K, Ogawa N, Ichihashi E, Kato H, Nagata Y, Ohtsubo Y, Tsuda M. 2018 Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon. Biosci Biotechnol Biochem, 82:1169-1171. Kishida K, Inoue K, Ohtsubo Y, Nagata Y, Tsuda M. 2017. Host range of the conjugative transfer system of IncP-9 naphthalene-catabolic plasmid NAH7 and characterization of its oriT* region and relaxase. *Appl Environ Microbiol, 83:02359-16 Tabata M, Ohhata S, Nikawadori Y, Kishida K, Sato T, Kawasumi T, Kato H, Ohtsubo Y, Tsuda M, Nagata Y. 2016. Comparison of the complete genome sequences of four γ -hexachlorocyclohexane-degrading bacterial strains: insights into the evolution of bacteria able to degrade a recalcitrant man-made pesticide. DNA Research*, 23:581-599
Activities in Academic Societies	Japan Society for Bioscience, Biotechnology, and Agrochemistry Society of Genome Microbiology, Japan

Recent Activities

In recent years, genome analysis has revealed the presence of plasmids carrying genes related to environmental pollutant degradation and antibiotic resistance. Plasmids are capable of transferring between bacterial cells through conjugative transfer. Bacteria that acquire these plasmids can become new environmental pollutant degraders or antibiotic-resistant strains. I am conducting research aimed at understanding the molecular mechanism of conjugative transfer with the goal of efficiently cultivating environmental pollutant-degrading bacteria and suppressing the emergence of antibiotic-resistant bacteria.

Message to Students