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川上浩一教授(国立遺伝学研究所)「ゼブラフィッシュにおけるトランスポゾンを利用した遺伝学的手法と機能的神経回路研究」

川上浩一教授(国立遺伝学研究所)「ゼブラフィッシュにおけるトランスポゾンを利用した遺伝学的手法と機能的神経回路研究」

第7回 発生ダイナミクス分野主催セミナー The transposon-mediated genetic methods in zebrafish and their applicat

2013.02.26 16:01
13:00~
片平 生命科学プロジェクト研究棟 1階講義室A
国立遺伝学研究所 初期発生研究部門 川上 浩一教授
生命科学研究科発生ダイナミクス分野 杉本 亜砂子

For the past decade, we have been developing genetic methods such as transgenesis, gene trapping, enhancer trapping and the Gal4FF-UAS system in zebrafish by using the Tol2 transposable element. By employing these methods, we have performed large-scale genetic screens, and generated more than 1,000 transgenic lines that expressed Gal4FF, an engineered Gal4 transcription activator, in specific cells, tissues, and organs. These transgenic fish are valuable resources for the study of developmental biology since these fish can be used to visualize the Gal4FF-expressing cells, and, further, to manipulate functions of the Gal4FF-expressing cells via the Gal4FF-UAS system.

We are interested in understanding cellular and molecular basis of animal’s behaviors.
Therefore we aimed to apply the Tol2-mediated methods to the study of functional
neural circuits. First we demonstrated that targeted expression of the neurotoxin gene
inhibits the Gal4FF-expressing neurons, thereby revealing the function of specific neural  circuits. Second, we demonstrated visualization of neuronal activity in spinal motor circuits during spontaneous contractions, and in tectal cells during perception of a natural object through targeted expression of the DNA-encoded calcium indicator GCaMP. Finally, we are currently studying leaning and memory using the adult zebrafish. We propose that gene trapping and the Gal4FF-UAS system in zebrafish are powerful approaches to dissect and identify functional neural circuits that control vertebrate behaviors.