Fluorescent proteins become indispensable tools in the field of lifescience to label intracellular molecules non-invasively. Variouscolors of fluorescent proteins are now available, which makes multicolorimaging possible. By applying the color variants to Föresterresonance energy transfer (FRET), we could design geneticallyencoded functional probes. The genetically encoded probes areapplicable for imaging in intact animal since it can be introduced bygenetic engineering method.
In addition to the color variation, we have found unique fluorescentproteins that show photoswitching property. These proteins serve asa fluorophore to track molecules and highlight cells on amicroscope. Recently, based on the photoswitchable fluorescentprotein, a new microscopic modality circumventing the diffractionlimit of light was invented. Here I introduce our recent achievementof functional imaging in intact animal and biological applications ofsuperresolution imaging.
This seminar will be held in English. セミナーは英語で行います