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北海道札幌市出身。東京、サンフランシスコベイエリア、仙台と巡ってきましたが、場所や使う生物や手法が変わっても一貫して細胞骨格とモータータンパク質の研究と、趣味の釣りをしてきました。
准教授 丹羽 伸介
| キャンパス | 青葉山 キャンパス |
|---|---|
| 所属研究室 |
神経細胞生物学
|
| 連絡先 | 022-796-4734 |
| shinsuke.niwa.c8＠tohoku.ac.jp | |
| ホームページ | https://www.fris.tohoku.ac.jp/~niwa/ |
| 経歴 |
2001 BSc, Department of Biology, School of Science, University of Tokyo.
2007 PhD, Department of Cell Biology, School of Medicine, University of Tokyo. Supervised by Dr. Nobutaka Hirokawa 2007 - 2012 Postdoctoral fellow, University of Tokyo. Supervised by Dr. Nobutaka Hirokawa 2012 - 2015 Research Associate, Stanford University. Supervised by Dr. Kang Shen 2016 - 2019 Assistant Professor, FRIS, Tohoku University 2019 - Current Associate Professor, FRIS, Tohoku University |
|---|---|
| 著書・論文 |
2025年
Naher, S., K. Iemura, S. Miyashita, M. Hoshino, K. Tanaka, S. Niwa, J.-W. Tsai, T. Kikkawa, and N. Osumi. 2025. Kinesin-like motor protein KIF23 maintains neural stem and progenitor cell pools in the developing cortex. The EMBO Journal. 44:331-355. Kita, T., R. Sugie, Y. Suzuki, and S. Niwa. 2025. Modular photostable fluorescent DNA blocks dissect the effects of pathogenic mutant kinesin on collective transport. Cell Reports Physical Science. 6: 102440 Kita, T., K. Sasaki, and S. Niwa. 2025. Biased movement of monomeric kinesin-3 KLP-6 explained by a symmetric Brownian ratchet model. Biophysical Journal. 124:205-214. 2024年 Niwa, S., T. Watanabe, and K. Chiba. 2024. The FHA domain is essential for autoinhibition of KIF1A/UNC-104 proteins. Journal of Cell Science. 137. Kita, T., and S. Niwa. 2024. Total Internal Reflection Fluorescence (TIRF) Single-Molecule Assay to Analyze the Motility of Kinesin. Bio-protocol. 14:e5135. Kita, T., K. Chiba, J. Wang, A. Nakagawa, and S. Niwa. 2024. Comparative analysis of two Caenorhabditis elegans kinesins KLP-6 and UNC-104 reveals a common and distinct activation mechanism in kinesin-3. Elife. 12:RP89040. Iguchi, R., T. Kita, T. Watanabe, K. Chiba, and S. Niwa. 2024. Characterizing human KIF1Bβ motor activity by single-molecule motility assays and Caenorhabditis elegans genetics. Journal of Cell Science. 137. Guo, X., C.H. Huang, T. Akagi, S. Niwa, R.J. McKenney, J.-R. Wang, Y.-R.J. Lee, and B. Liu. 2024. An Arabidopsis Kinesin-14D motor is associated with midzone microtubules for spindle morphogenesis. Current Biology. 34:3747-3762. e3746. Chiba, K., and S. Niwa. 2024. Autoinhibition and activation of kinesin-1 and their involvement in amyotrophic lateral sclerosis. Current Opinion in Cell Biology. 86:102301. 2023年 Niwa, S., and K. Chiba. 2023. Generation of recombinant and chickenized scFv versions of an anti‐kinesin monoclonal antibody H2. Cytoskeleton. 80:356-366. Kita, T., K. Sasaki, and S. Niwa. 2023. Modeling the motion of disease-associated KIF1A heterodimers. Biophysical Journal. 122:4348-4359. Higashida, M., and S. Niwa. 2023. Dynein intermediate chains DYCI‐1 and WDR‐60 have specific functions in Caenorhabditis elegans. Genes to Cells. 28:97-110. Chiba, K., T. Kita, Y. Anazawa, and S. Niwa. 2023. Insight into the regulation of axonal transport from the study of KIF1A-associated neurological disorder. Journal of Cell Science. 136:jcs260742. 2022年 Taguchi, S., J. Nakano, T. Imasaki, T. Kita, Y. Saijo-Hamano, N. Sakai, H. Shigematsu, H. Okuma, T. Shimizu, and E. Nitta. 2022. Structural model of microtubule dynamics inhibition by kinesin-4 from the crystal structure of KLP-12–tubulin complex. Elife. 11:e77877. Nakano, J., K. Chiba, and S. Niwa. 2022. An ALS‐associated KIF5A mutant forms oligomers and aggregates and induces neuronal toxicity. Genes to Cells. 27:421-435. Imasaki, T., S. Kikkawa, S. Niwa, Y. Saijo-Hamano, H. Shigematsu, K. Aoyama, K. Mitsuoka, T. Shimizu, M. Aoki, and A. Sakamoto. 2022. CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation. Elife. 11:e77365. Chiba, K., K.M. Ori-McKenney, S. Niwa, and R.J. McKenney. 2022. Synergistic autoinhibition and activation mechanisms control kinesin-1 motor activity. Cell reports. 39. Anazawa, Y., and S. Niwa. 2022. Analyzing the impact of gene mutations on axonal transport in Caenorhabditis elegans. In Axonal Transport: Methods and Protocols. Springer US New York, NY. 465-479. Anazawa, Y., T. Kita, R. Iguchi, K. Hayashi, and S. Niwa. 2022. De novo mutations in KIF1A-associated neuronal disorder (KAND) dominant-negatively inhibit motor activity and axonal transport of synaptic vesicle precursors. Proceedings of the National Academy of Sciences. 119:e2113795119. 2021年 Takahashi, H., M. Kamiya, M. Kawatani, K. Umezawa, Y. Ukita, S. Niwa, T. Oda, and Y. Urano. 2021. Neural and behavioral control in Caenorhabditis elegans by a yellow-light–activatable caged compound. Proceedings of the National Academy of Sciences. 118:e2009634118. Lam, A.J., L. Rao, Y. Anazawa, K. Okada, K. Chiba, M. Dacy, S. Niwa, A. Gennerich, D.W. Nowakowski, and R.J. McKenney. 2021. A highly conserved 310 helix within the kinesin motor domain is critical for kinesin function and human health. Science advances. 7:eabf1002. Hayashi, K., M.G. Miyamoto, and S. Niwa. 2021. Effects of dynein inhibitor on the number of motor proteins transporting synaptic cargos. Biophysical Journal. 120:1605-1614. 2020年 Monroy, B.Y., T.C. Tan, J.M. Oclaman, J.S. Han, S. Simó, S. Niwa, D.W. Nowakowski, R.J. McKenney, and K.M. Ori-McKenney. 2020. A combinatorial MAP code dictates polarized microtubule transport. Developmental cell. 53:60-72. e64. Hayashi, K., S. Hasegawa, and S. Niwa. 2020. What is the temperature of a cell? Europhysics News. 51:48-50. 2019年 Yanagi, S., T. Sugai, T. Noguchi, M. Kawakami, M. Sasaki, S. Niwa, A. Sugimoto, and H. Fuwa. 2019. Fluorescence-labeled neopeltolide derivatives for subcellular localization imaging. Organic & Biomolecular Chemistry. 17:6771-6776. Shao, Z., S. Niwa, A. Higashitani, and Y. Daigaku. 2019. Vital roles of PCNA K165 modification during C. elegans gametogenesis and embryogenesis. DNA repair. 82:102688. Hayashi, K., S. Matsumoto, M.G. Miyamoto, and S. Niwa. 2019. Physical parameters describing neuronal cargo transport by kinesin UNC-104. Biophysical reviews. 11:471-482. Gabrych, D.R., V.Z. Lau, S. Niwa, and M.A. Silverman. 2019. Going too far is the same as falling short†: Kinesin-3 family members in hereditary spastic paraplegia. Frontiers in Cellular Neuroscience. 13:419. Chiba, K., H. Takahashi, M. Chen, H. Obinata, S. Arai, K. Hashimoto, T. Oda, R.J. McKenney, and S. Niwa. 2019. Disease-associated mutations hyperactivate KIF1A motility and anterograde axonal transport of synaptic vesicle precursors. Proceedings of the National Academy of Sciences. 116:18429-18434. |
| 所属学会 |
American Society for Cell Biology, 日本細胞生物学会、日本分子生物学会、日本解剖学会 |
最近の研究について
シナプスの材料を軸索輸送する分子モータータンパク質であるKIF1Aの遺伝的変異は運動神経障害、自閉症、発達障害、学習障害といった先天性の脳神経疾患の原因となります。ゲノム編集を用いて疾患モデル線虫を作成し、この疾患にはKIF1Aのモーター活性が低下し軸索輸送が減る場合と、KIF1Aのモーター活性が亢進して軸索輸送が増加する場合の2つのタイプがあることを発見しました (PNAS, 2019とPNAS, 2023)。モーター活性の亢進はKIF5Aという別のモータータンパク質の遺伝子の変異を原因とするALSでも起こっていました (Genes Cells, 2023)。KIF1Aの活性が亢進するときにはKIF1Aの二量体化が促進されていることを明らかにしました (eLife, 2024)。複数のモータータンパク質の協調運動をin vitro再構成で簡単に観察するために、DNAオリガミを用いた手法を開発し、FTOBと名付けました (Cell Rep Phy Sci, 2025)。